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Rice plants are grown in pots and kept in a growth chamber ranging from 22 to 26 °C with the cycle of 14 h light/10 h dark.
Plant debris was mixed with non-sterile soil at a ratio of 1 4 by weight, placed in pots, and kept in a growth chamber, same conditions as described above.
Seedlings were planted into 4L pots and kept in a greenhouse at 25°C.
Two months after rooting, the plantlets were planted in the soil pots and kept in a growth chamber for further growth.
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The germinated seedlings were sown in pots containing soil and kept in a growth chamber.
Commercial sugarcane cultivars, Saccharum spp. hybrids, Q138, Q157 and Q179A were grown from setts (stem cuttings) in coarse perlite within 15 cm diameter free-draining pots (2 L), and kept in a naturally lit glasshouse for 3 months.
Seeds were sown in 294-cm3 pots filled with a peat moss mix and kept in a greenhouse for 2 weeks.
Nodal cuttings with 2 3 pairs of leaves were excised from the runners and planted in half-strength hydroponics Hoagland's medium (Hoagland and Arnon 1950) in plastic pots (diameter × height: 21 × 7 cm) and kept in a growth chamber with a dark/light (300 350 µmol m−2 s−1 of photosynthetically active radiations) cycle of 16/8 h at 25 °C.
For aphid fitness experiments, plants were sown directly to pots with soil (one plant per pot) and kept in chambers under a 16/8 h (light/dark) photoperiod.
Ten seeds were sown manually at equidistant positions and seedlings were thinned to three plants per pot and kept in the nursery throughout the experiment.
Seeds were planted in plastic pots with sterilized soil and kept in the greenhouse at a temperature of 25-30°C 25-30°C
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