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In order to avoid potential unspecific effects of pharmacological PKC delta inhibition, we isolated EPC from the spleen of PKC delta deficient mice or wild type mice.
To test for potential unspecific effects of the 7PA2 supernatant, we immunodepleted A β from the 7PA2 conditioned medium by using the anti-A β monoclonal antibody IC16.
To precise the role of p53 in ESC differentiation and to avoid potential unspecific effects of chemical compounds, we then used two genetic approaches: transient transfections of anti-p53 siRNAs and analysis of differentiation capacities of ESC knockout for the p53 gene.
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However, we cannot discount possible unspecific effects of task order.
In addition, the nanoclays suspensions were sonicated in order to reduce any possible unspecific effect of the NC aggregation.
Putative mechanisms involved in interactions between AM fungi and organics include direct effects of enzymes secreted by hyphae, and indirect effects of enhanced root-derived enzymes, microbial activity and modified microbial composition, and unspecific effects of changes in pH, osmotic potential, red-ox potential, partial pressures of O2/CO2 [26], [27], [29], [30], [30].
In order to test the effect of nuclear maspin on normal cell proliferation and potential unspecific cytotoxicity for normal breast cells we chose the epithelial normal cell line MCF10A.
One of the most prevalent unspecific effects is triggering the innate immune system in mammals.
The inclusion of such group is crucial to control for unspecific effects.
Moreover, we discuss implications for future clinical trials and present novel study designs enabling researchers to consider the complex interplay of drug-specific and unspecific effects.
Since this tool is known to also have unspecific effects, control experiments are needed, chiefly among them the exclusion of off-target effects and the reconstitution of the genes' expression for the rescue of the cellular RNAi effects.
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