This potential promoter was designated as pSH4.
The potential promoter regions recognized by Sigma factor D (σD) were predicted using prediction of bacterial promoters (BPROM) provided by Softberry Inc. (http://www.softberry.com).com
With consideration for potential promoter interruption and the demonstrated efficacy of intronic insertions, 61%% of mapped T-DNA insertions should impair gene expression or function.
To achieve the objectives, we produced transgenic rice plants containing a chimeric gene construction with the potential promoter region of SH4 fused with the GUS gene, and examined the expression pattern of GUS activity.
Application of this model to a test set of core promoters not only gave better discrimination of potential promoter sites than previous statistical or neural network models, but also revealed indirectly subtle properties of the transcription initiation signal.
Interestingly, for both SV7 and SV8, a specific potential promoter region could be identified.
Thus, it was possible that potential promoter activity from the ZNF 3' exons was suppressed due to KAP1-mediated repression.
According to this study both SNPs are potential promoter polymorphisms and located in a region important for transcription regulation.
Nucleotide sequence analysis of region upstream to the sodC gene identified a single-nucleotide insertion in the potential promoter region.
In addition, databases searches for putative promoter regions in the mouse prominin-1 gene revealed three potential promoter regions that could be linked to specific SVs.
A potential promoter was found near the start of the rfbB region, supporting the possibility that the rfb gene cluster was an operon.
