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The growing potential of sequencing to carry out transcriptomics has been recently demonstrated by reports of this technology being used at the single cell level [14].
Within a decade this will help make cancer a chronic disease, as opposed to an often fatal one.Everything in sequenceMany new groups will catch on to the potential of sequencing in 2015.
However, 18 short human sequences were closely related to the HBRV LTR and even accounting for the potential of sequencing error, these data suggest that the vast majority of any human endogenous retroviral sequences amplified by the LM-PCR process would be discarded by the pipeline (calculated as less than 1.4 × 10−7).
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A greater understanding of the limitations and potential pitfalls of sequencing technology is essential before clinical uptake of this technology [ 4].
During the assembly process, only reads with a 100% match in the overlapped region were assembled, eliminating potential effects of sequencing errors on assembly.
A quality control step was included in our workflow to detect potential cases of sequencing errors due to contamination or paralogy.
In this study, the use of molecular barcoding allowed multiplexing without loss of individual sequence information, which is required to fully exploit the potential of sequence data.
The listeriosis outbreak described clearly underlines the potential of sequence-based typing methods to offer enhanced resolution and comparability of typing systems for public health applications.
Robust designs of PCR-based molecular diagnostic assays rely on the discrimination potential of sequence variants affecting primer-to-template annealing.
Jarek Meller (University of Cincinnati) discussed the potential of sequence-based solvent accessibility prediction for mapping RNA interaction sites in proteins.
Assigning proteins to a large GH-family, like GH5, which harbors multiple specificities and activities, does not unlock the full potential of sequence-based classification.
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