We initially identified a potential nuclear localisation signal (NLS) within the unique N-terminal domain of BAG-1L, consistent with the predominantly nuclear localisation of this isoform (Packham et al, 1997; Takayama et al, 1998; Yang et al, 1998; Brimmell et al, 1999).
In addition, ERK8 has been reported to localise perinuclearly in A431 cells (Uhlen et al., 2010), suggesting a potential Golgi localisation.
The expression of each tagged protein was induced for 2 h with CuSO4 followed by a 2 h chase sometimes in the presence of cycloheximide that was included during chase to minimize potential false localisation of transmembrane proteins in the ER.
The total predicted core and accessory proteome of C. diphtheriae was further analysed to determine potential functional localisation.
Nevertheless, this protein has been found to interact with the C-terminus of aquaporin 5 suggesting a potential cytoplasmic localisation [ 52].
However, intracellular aggregates of mature Aβ42 were elusive in vaccine formulations adjuvanted with Adju-Phos® in which the potential co-localisation of amyloid fibrils and ABA could only be detected at higher magnifications.
There is potential for regulated localisation, since acetylation of the nuclear localisation signal of IFI16 led to its cytosolic accumulation [ 31].
The photoluminescence (PL) properties are governed by lateral energy transfer within a dense ensemble of quantum dots, which differ in size and Cd concentration, providing for a complex potential landscape with localisation sites of widely varying depth for excitons.
Thus, while it is possible that these potential 'detection without localisation' trials in the low-density condition could reflect a global signal that is used to make a detection response, as proposed by Evans et al. (2013, 2016), these trials could alternatively reflect the contribution of other factors to reducing localisation accuracy.
Lysosomes have been shown to be a potential site of localisation for aggregated and/or hydrophilic sensitizers (Gèze et al, 1993; Berg and Moan, 1997).
