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In both the tripotent and bipotent states, pluripotency has been lost but some limited tissue-specific regenerative potential is retained.
It is now thought that monocyte potential and indeed granulocyte potential is retained by primitive multi-lymphoid lymphoid or lymphoid-primed progenitors (Goardon et al, 2011; Doulatov et al, 2012) and the first fate decision is more accurately defined as the separation of megakaryocyte and erythroid potential from 'nucleated cell potential' (Arinobu et al, 2007; Sanjuan-Pla et al, 2013).
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However, we find that conserved structural features with conserved bonding potential are retained to maintain this binding phenotype.
This adherent colony-forming potential was retained by cells isolated at 10·5 dpc but lost on extended culture of 11·5 dpc cells.
The inability of FOG-1-deficient HSCs to produce Mk/E colonies and give rise only to GM myeloid colonies, combined with the myeloid reprogramming of FOGcKO preMegEs raised the question whether GM potential was retained in this phenotypically Mk/E-committed cell population.
In this issue of EMBO Molecular Medicine, Rehe et al (2013) build on earlier work in which they demonstrated that in high-risk B-ALL, leukemogenic potential was retained in CD34+/CD19− (the least mature subset as judged by immunophenotype), CD34+/CD19+ and CD34−/CD19+ populations (le Viseur et al, 2008).
Entry of extracellular free Ca2+ through voltage gated Ca2+ channel on astrocytes is also unlikely because astrocytic membrane potential in vivo is retained below −75 mV under urethane anesthesia as we reported recently using in vivo intracellular recording method [35] [37].
Using a recently developed simulation technique, we allow full equilibrium, i.e. the chemical potential of the polyelectrolyte is retained as the separation is changed.
We also show that the potential to change color is retained until the final (sixth) larval instar.
The results of fish with switched rations showed that the potential for growth compensation is retained during the most of the juvenile period.
These findings suggest that the cause of rapid washout of 99mTc-MIBI is the loss of mitochondrial membrane potential since 99mTc-MIBI is retained to the mitochondrial membrane in response to membrane potential.
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