For SodB that has been identified as a factor in F. tularensis oxidative stress protection [ 71], we found multiple potential interacting partners, including interactions with the murine thioredoxin-interacting protein Txnip, a protein mediating oxidative stress via thioredoxin activity [ 72].
Three chemometric approaches were applied: (i) factorial design (33 applied to surface plot and 23 applied to effect factor) for screening the potential interacting variables, (ii) univariant for optimizing insignificantly interacting variables and (iii) simplex for optimizing potentially interacting variables.
We have identified RanBPM as a potential interacting protein and confirmed this interaction by in vitro assay.
Apart from the molecular dissection of known interactions and the identification of new potential interacting partners, several new problems can now be assayed by use of two-hybrid based technology.
By examining correlated expression patterns, we can hypothesize new functions for previously uncharacterized structured transcripts or identify potential interacting RNA molecules as well as RNA-protein interactions due to localized translation as described above.
To search for negative regulators of STAT3, we examined the possibility of its interaction with different phosphatases, and PTPMeg2 was identified as a potential interacting protein.
We looked for AChE structural data useful for in silico design of potential interacting molecules.
This study investigated the potential interacting influences of allelopathy and resource competition on plant growth density relationships.
Particularly, the high ranking of hERG as a potential interacting target of a query compound may serve as a hERG toxicity alert for further safety investigation.
A plethora of potential interacting partners have been identified, particularly for the secreted form of NS1, with many being implicated in immune evasion strategies.
Ligand-based in silico target fishing can be used to identify the potential interacting target of bioactive ligands, which is useful for understanding the polypharmacology and safety profile of existing drugs.
