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To facilitate the potential insertion of additional HA epitopes, a NheI restriction site was inserted at the 3′-end of each HA sequence, accounting for the additional S residue found at the C-terminus of each HA tag.
A 2D Boundary Element Method gives predictions that agree well with data for parallel wall arrays up to 16 m long and it is used to investigate the potential insertion loss of longer configurations up to 0.3 m high.
We report the approach to overcome the deterrents of the hexagonal Li2.6Co0.4N as potential insertion anode for lithium ion batteries: the rapid capacity fading upon long cycles and the fully Li-rich state before cycling.
These alignments could then be examined for mismatching bases and alignment gaps, with mismatches indicating potential miscalled bases, gaps in the ENCODE sequences indicating potential insertion errors, and gaps in the 2× assemblies indicating potential deletion errors.
Potential insertion mutations were detected using split-end alignment (Smith 2011).
Although this estimate falls within our confidence interval, our results suggest, generally, a denser population of potential insertion sites.
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Local Realignment around potential insertion-deletion sites and base quality scores recalibration were implemented using GATK [ 37, 38] and fix mate-pair information using SAMtools [ 36] to improve the quality of the sequence data before variant calling.
The models included potential insertions and deletions.
Primers flanking the predicted insertions fragment were generated and the potential insertions were validated by polymerase chain reaction (PCR) amplification.
Any potential insertions or deletions (indels) were investigated by analysis of the flowgrams in the GS program suite.
Thus, these methods require pooling of multiple experiments using different restriction enzymes or primers to come close to saturating the potential insertions.
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