The utilization of human NSCs as a potential source for reprogramming into iPS cells offers multiple advantages.
Eminli, S. et al. Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cells.
Embryonic cells have great potential for cellular reprogramming, and cellular reprogramming through epigenetic modifications and changes in transcription are associated with regenerative responses [ 7].
We hypothesized that genetic reprogramming may be improved by enhancing the transactivation potential of reprogramming factors through protein engineering.
While our network includes several factors that have been used successfully for reprogramming, it also includes other potential factors that might contribute to this process and may warrant further investigation.
Collectively, these results demonstrate that p53−/− MEFs are more efficiently reprogrammed to hematopoietic progenitors with erythroid, myeloid, megakaryocyte, and B and T lymphoid lineage potential than wild-type MEFs, indicating that P53 expression is a barrier for reprogramming to blood.
The high expansive potential of pSSCs in vitro and their intrinsic expression of Sox2 and Oct3/4 suggest pSSCs as interesting candidate cells for reprogramming into iPSCs.
Although the derived iPSC lines could be useful for lung disease modeling, the use of a viral vector for reprogramming and the part of DNA sequence left behind at unknown site(s) after removal of transgenes are potential drawbacks.
The techniques for reprogramming are far from optimized; mutations during reprogramming may cause abnormalities in the iPSC lines; and the differentiation potential of iPSC lines may vary.
