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Considering the balance between the LE and the potential cell damage due to prolonged glucose deprivation, the duration of glucose deprivation was set at 60 min in subsequent studies.
It was hypothesized that, because of their short tissue range, Auger/conversion electrons are unable to damage the radiosensitive glomeruli and potential cell damage would, therefore, be limited to the more radioresistant tubular cells, in which the radioconjugates accumulate [26].
Both of these approaches have limitations in systematic siRNA-mediated screening experiments, including the potential cell damage and equipment and reagent costs associated with electroporation in a high-throughput format or the complexity and safety issues associated with virally-mediated transfection.
Given that As-HAE is the most used in folkloric medicine, its administration should be controlled in order to prevent potential cell damage.
However, many environmental pollutants can also induce ROS formation through an endogenous mechanism to alert for potential cell damage and increase environmental stress tolerance [ 11, 12].
Owing to its anatomical location and function, RPE is continuously exposed to potential cell damage caused by oxidative stress, specifically due to oxygen and nitrogen reactive species.
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Consequently, potential cell damages can unambiguously be related to particle and gas exposure.
The loss of the pathway may also be more directly advantageous to bacteria by reducing the potential of cell damage from reactive oxygen species produced by the accumulation of heme (Anzaldi and Skaar 2010).
It should again be highlighted that by using slow-thawing conditions, we are maximising potential for cell damage to provide a rigours test – higher levels of recovery could be achieved with fast thawing (∼80%) but our aim is to eliminate all recrystallization associated damage.
Hydroxymethylfurfural (HMF) and alpha-ketoglutaric acid (KG) have been recently investigated as potential cancer cell damaging agents.
The amount of damage to host cells has been shown to be directly proportional to the concentration of the sodium hypochlorite solution; thus, the higher the concentration of sodium hypochlorite, the higher the potential for host cell damage.
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