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A total of 17 unigenes homologous to the T13αH gene and one unigene homologous to the TBT gene were detected in the C. cladosporioides MD2 transcriptome, and this branch participated by T13αH and TBT might be a novel and potential branch for the 10-deacetyl-2-debenzoylbaccatin III biosynthesis of C. cladosporioides MD2.
HSF analysis confirmed disruption of the acceptor-splice site and predicted that the potential branch point of intron 6 would be broken.
Our data reveal the critical importance of just one of three potential branch points, the polypyrimidine tract and the splice acceptor sequence in the maintenance of normal exon five splicing.
Every 5-nucleotide sequence within the window was evaluated as a potential branch point.
This identified potential branch points in each intron and generated alignments of these and the 5'/3' splice sites.
Rather than maximizing sequences per species, we concentrated on increasing taxon sampling to minimise potential branch lengths.
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Executives brought landlords for potential branches to existing locations to prove Citi was a legitimate outfit.
The criterion of a modified hoop stress intensity factor, which accounts for fracture toughness anisotropy, is applied to predict potential branching directions.
However, due to anatomical variability and unpredictability, treating the entirety of the treatment line(s) in all patients may be worthwhile to ensure that all potential branches are targeted.
However, in the C. membranifera introns these pyrimidine-rich sequences include the potential branch-point sequences whereas in T. vaginalis these sequences (usually T-rich) are found upstream of the potential branch-point sequences.
They contain a consensus 5' splice site sequence GTAYGT and, based on constraints imposed in the search parameters, the 3' splice-site sequence ACTAAC ACAYAG, where the nucleotides in bold italics indicate the potential branch-point sequence.
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