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The resulting cumulative reducing potency is assessed with the controlled-current EC method, and this parameter is probably more relevant for characterizing an AOX than E1/2 of a single redox system only.
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The stock formulation was then harvested and potency was assessed by HPLC/DAD, and solid state checked by Powder X-ray diffraction (PXRD).
Six selected candidates from the screening were then synthesized and their inhibitory potency was assessed both in vitro and ex vivo.
OCP migration to the SDF-1 gradient and the osteoclast forming potency were assessed in chemotaxis/osteoclastogenic assays.
Therefore, GR transactivation potency was assessed in the GeneBLAzer β-lactamase reporter gene technology in MMTV-bla HeLa CellSensor® cells (Invitrogen).
Potency was assessed as previously described 34, 35 by quantification of late liver stage parasites expressing Pf merozoite surface protein 1 (PfMSP-1) 37 in cultured human hepatocytes (HC-04 cells) 38 6 days after addition of PfSPZ (Table 1, Supplemental Figure S2).
Potency was assessed in two flow cytometry-based, cell-based assays: the binding of a PE-labeled CD40 Fc fusion protein to CD40L-expressing D1.1 Jurkat cells and an assay of T-cell-dependent B cell activation, involving overnight co-culture of CD40L-expressing D1.1 cells with the Ramos B cell line and monitoring intercellular adhesion molecule 1 (ICAM-1) expression on the latter cells.
The HCl/Ethanol method is a rapid and convenient way of screening agents of antiulcer potency, which is assessed in terms of absence or reduction in macroscopically visible lesions [ 29].
The potency of antivenoms is assessed by analyzing the neutralization of venom-induced lethality, and is expressed as the Median Effective Dose (ED50).
The analogy between the originator and its biosimilar(s) is assessed through safety, purity, and potency analyses.
Selectivity is assessed based on pair-wise compound potency ratios.
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