We previously established CbCln3Δex7/8 neuronal precursor cell lines by conditionally immortalizing cerebellar granule neurons from postnatal wild-type, heterozygous or homozygous littermate Cln3Δex7/8 mice [20].
Briefly, astrocytes in primary cultures were prepared as previously described (Araujo et al, 1993) from 1-day postnatal wild-type C57BL/6J mice.
NPCs were explanted from the brains of postnatal wild-type, Id2+/− and Id2−/− mice and propagated as described (Reynolds and Weiss, 1992).
DOI: http://dx.doi.org/10.7554/eLife.01323.018 10.7554/eLiFigure2 figureigure 2—figure supplement 8. Electrocardiogram tracings of a postnatal wild-type or miR-1 null animal on a mixed background.
Second, transplantation techniques have been developed that allow transplantation of mammary epithelium or intact mammary glands from embryonic and postnatal sources into wild type host animals.
Brain hemispheres of postnatal day 10 14 wild type or transgenic Arl13B-EGFP tg mice (Delling et al., 2013) were sectioned with a vibratome (Leica) to 200 μm slices in an ice-cold oxygenated solution containing in mM: 87 NaCl, 25 NaHCO3, 1.25 NaH2.54, 2.5 KCl, 75 sucrose, 25 glucose, 7.5 MgCl2.
Expression of pachytene-induced spermatogenesis genes at postnatal day 11 and day 18 of testis development [ 46], and at postnatal day 16 in wild type and Ubr2 knockout testis [ 44].
Genome-wide microarray analysis was used to identify differences in the gene expression profiles of late gestation and early postnatal total pancreas tissue from wild type and Hnf6 transgenic animals.
To investigate if the identified body weight differences detected at birth would persist in postnatal life we next analysed wild type, Grb10 m/+, Dlk1 +/p and Grb10 m/+ /Dlk1 +/p mice at one week, three to six months and more than six to nine months of age.
Primary cortical neurons were prepared from wild type pups (1-2 postnatal days).
The LD muscle also undergoes postnatal hypertrophy in callipyge (NCpat) compared with wild type (NN) sheep [12], [16], [17], [18].
