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The statue currently stands on a closely cropped lawn in front of one of the company's cookie-cutter corporate buildings, his gilded posterior level with a row of windows.
To establish the percentage of new-generated PGCs and GCs double-labelled for the different markers, serial sections from standard, enriched and deprived mice were examined systematically through the OB (3 sections positioned respectively at antero, medial and posterior level).
GAD67 signal intensity was measured by densitometry using ImageJ software (NIH, Bethesda, MD) on 3 sections (positioned respectively at antero, medial and posterior level) per animal and corrected to the background signal.
To calculate the total number of TH- positive cells in the GL, we applied a random sampling method on 3 sections (positioned respectively at antero, medial and posterior level) per animal; density and total number of TH- positive cells were calculated as described above.
In mice initiation of closure of the optic fissure has been reported to occur at a posterior level close to the developing optic disc rather than from a central midpoint in a proportion of animals (35).
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They found that, despite a significant lower increase of CK level with a "minimally invasive" anterior approach compared with posterior, levels of interleukins did not show significant differences between the two groups.
The V4 activation can be observed in all 3 monkeys at more posterior levels: −11 in M3 and M5 and −13 in M1.
Of note, at the difference of PCNA, Musashi, Notch and Delta expression did not show obvious discontinuities in the A/P axis of the neural tube -although some patches and sub-domains may be observed for Musashi and Pisolo in the D/V axis at posterior levels.
There was clearly strong up-regulation of proTRH immunostaining in both striosomes and matrix in the most intensely stained zones of the caudoputamen, but as the proTRH immunostaining diminished medially, the staining was uneven, and at posterior levels, the small zones of intense immunostaining had the appearance of striosomes.
In the pulvinar, dense labeling occurred at posterior levels.
At slightly more posterior levels, additional label was found in area 32.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com