Lysozyme C mRNA expression is confined to blood and posterior kidney only whereas lysozyme G mRNA is expressed in all tissues.
Similarly, tissues such as muscle, ovary, heart, brain, gill, testis, liver, intestine, spleen, skin, posterior kidney, anterior kidney and blood collected from adult brown trout were subjected to detection of all selected genes by RT-PCR.
intestine, heart, ovary, gill, spleen, muscle, liver, brain, skin, anterior kidney, posterior kidney, and blood collected from juveniles of rohu were subjected to detection of all above mentioned gene transcripts by RT-PCR.
Fish were thawed until pliable and tissue samples (spleen, liver, anterior and posterior kidney, heart) were collected aseptically, with sterile forceps and dissection scissors, disinfected scalpel blade handle and sterile scalpel blade for every individual fish.
Specimens from a fish that died 3 days postchallenge were cultured on SBA, and GBS was recovered from nostrils, intestines, posterior kidney, and brain.
The teleost anterior kidney is a main site for B lymphogenesis, while the teleost posterior kidney provides an environment capable of inducing B cell activation and differentiation into plasma cells [ 45].
T is the anterior-posterior body thickness at the kidney level, and x is the anterior-posterior kidney thickness.
In addition to the ovary, aqp1b mRNA has been detected in the posterior intestine, kidney, gills and esophagus of marine fish (this work, and [ 10, 14, 38]).
Total RNA was extracted from different tissues, ovary, testis, brain, eye, gills, anterior intestine, midgut, posterior intestine, kidney, liver, muscle and skin, of 5-10 adult fish using the RNeasy Mini Kit (Qiagen).
The following tissues were collected from goldfish: hindbrain, midbrain, hypothalamus, telencephalon, olfactory bulbs, pituitary, anterior intestine (J-loop), posterior intestine (rectum), kidney, spleen, liver, heart, eye, muscle, gill, gall bladder, adipose, ovary, testes, and skin.
In rodents, detailed analysis of SLC4A11 mRNA and protein expression indicated measurable abundance in anterior and posterior corneal epithelia, kidney, stomach, duodenum, pancreas, brain and choroid plexus epithelium (42).
