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The positive FFR in 4 Hz stimulated EHM was associated with reduced calcium sensitivity, frequency-dependent acceleration of relaxation, and enhanced post-rest potentiation.
Manipulating intracellular Ca2+ with various positive inotropic interventions (e.g. post-rest potentiation, isoproterenol) and changes in stimulus frequency demonstrated that mechanical properties can be improved in subsets of myocytes.
Muscle experiments in vitro revealed impaired contractile force and post-rest behavior.
Ran did not affect post-rest behavior in TG papillary muscle (Fig. 4b).
A different experimental protocol which is thought to enable increased Ca2+ accumulation into the SR stores in diastole, uses post-rest pacing which typically produces an increase in developed force in healthy myocardium also referred to as 'post-rest potentiation'.
However, alterations in post-rest potentiation that may underlie reduced stress adaptation and the FFR inversion have been associated with a blunted post-rest ∆[Ca2+]i increase of SR Ca2+ content (Fig. 2) [ 80].
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In human failing cardiac muscle strips we typically observe a robust post rest potentiation (Fig. 3b).
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