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These analyses support the idea that in head tissues a shift in metabolic state, nutrient utilization, and storage is involved with the post-mating response as early as 24 hours post-mating.
RNA was derived from age-matched, adult head tissues from virgin and mated females at 0-2, 248 48, and 72 hours post-mating, and from brain tissues at 24 hours post-mating.
To more specifically identify genes that play a role in the CNS to mediate post-mating behavioral changes, gene expression analyses were performed on dissected brain tissues 24 hours post-mating.
Of the 28 genes that are repressed in dissected brain tissues 24 hours post-mating, only one gene, Obp99b, was also identified as repressed in whole head tissues 24 hours post-mating.
Also, serum P4 concentration was determined weekly throughout 28 days post-mating.
AAEL010824 levels in a depleted male are replenished by 48 h post-mating.
Serum P4 concentration increased (P < 0.05) on Day 14 post-mating and continued higher (P < 0.05) until Day 28 post-mating in the GnRH7 and GnRH0+7 groups compared with the control.
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Forty-seven genes differed between mated and control heads 2 hrs post mating.
Levels of fit are elevated 2 hrs post mating (G.E.C. and L.L. Ellis, unpublished results).
Alternatively, post mating sexual selection may also favour males with competitive ejaculates to sire more competitive offspring [ 14].
These species are reproductively isolated by partial ecological isolation [ 21], sexual isolation and post mating barriers [ 22].
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