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Numerous interruptions in the line representing the duplicated region indicate that there has been significant post-duplication deletion/insertion related modification of the duplicated region.
One more cross-species study reports that most duplicated genes experience a short post-duplication period of relaxed neutral selection [ 12].
The ancestral gene order for the pre-duplication ancestor (n = 7) consists in only remaining duplicated genes characterized in the post-duplication ancestor (n = 12).
Therefore, in these three cases, the duplicated Dlx genes have had very different post-duplication fates.
Further, as repression mediated by microRNAs is typically weak [ 38], so that post-duplication dosage is likely to be above ancestral levels, duplicability biases might be rather subtle.
Post-duplication evolutionary rate acceleration has been revealed primarily through sequence comparisons between duplicated genes.
The post-duplication acceleration has the effect of mitigating the sequence divergence differences between duplicates and singletons.
The case is somewhat more complicated for two post-duplication outgroups.
We believe this is a consequence of post-duplication gene conversion events within the genus Anableps.
Thus in this case: (i) a post-duplication outgroup yields shorter average internal branches, and (ii) later-diverging post-duplication outgroups yield shorter internal branches than do more distant ones (i.e. small values of e).
By contrast, all cases with at least one post-duplication outgroup (Figure 2c,d and Figure 3b e, excepting post-duplication pairs for which e1 = e2) contain a KNST branch, thus outgroup combinations that include at least one post-duplication outgroup are preferred.
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