The perylene quencher does not affect the activity of nuclease, which makes it possible to design post-addition type bioassay platform.
The results showed that 16 18 h post-addition, the surfactants exhibited different degrees of toxicity.
The signal at each wavelength in a given scan was decay corrected for time post-addition of activity to the cuvette.
LLVY was added (13 µM final concentration), and florescence measurements (excitation/emission: 355/460 nm) were taken at 30, 60, 90, and 120 minutes post-addition using a Perkin-Elmer Victor3 1420 multilabel counter.
Direct and indirect measurements of DO post-addition of AA were performed using a DO meter (CyberScan DO300 Waterproof DO Meter, Eutech Instruments, Singapore) with a portable probe and methylene blue (MB) redox dye, respectively.
At 0, 24, and 48 h post-addition of chemotherapeutic, cell viabilities in cultures were determined by addition of 3- 4,5-dimethylthiazol-2-yl -5- 3-carboxymethoxyphenyl -2- 4-sulfophenyl -2H-tetrazolium (MTS) cell proliferation reagent, incubated for 3- 4,5-dimethylthiazol-2-yl -5- 3-carboxymethoxyphenyl -2- 4-sulfophenyl -2H-tetrazolium
The results of the HOS test obtained 1 h post-addition of the surfactants showed that at CMC/2 all surfactants except Triton X-100, C14TAB and N-9 were able to affect the integrity of the plasma membrane of the sperm cells (Table 6).
Assays were run for 5 days post-addition of extract without any change of medium.
The scratch assay disclosed an enhanced ovarian cancer cell migration at 12 and 24 hours post-addition of platelets.
Viability of both strains was unaffected for 4 h post-addition of iCORM-3 (Supplementary Fig. S3).
Following nitrogen addition, triplicate cultures were harvested at 4, 12, 24, and 48 h post-addition and total RNA extracted.
