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For in vitro culture experiments, the pollinated styles were excised 5 hours post pollination and inoculated on slides with a solid medium containing 100 mg/L H3BO4, 300 mg/L CaCl2, 10% sucrose and 1% agarose.
14 15 days post pollination, the immature grains were collected and sterilized with 70% ethanol for 1 min, immersed in 20% bleach for 15 min and rinsed with sterile distilled water for five times inside laminar flow bench.
Signaling from pollen to style has been inferred from changes in flower morphology that become apparent by two days post pollination, where the petals close up and prepare to drop off allowing the fruit to develop unencumbered (Figure S1).
Ears were harvested at 5 6 weeks post pollination.
Classically, fleshy fruit development is described to consist of three stages post pollination: cell division, cell expansion, and ripening [ 1].
File 2. mRNA-seq-based expression of the different apple gene models in mature 'Royal Gala' apples and MADS8/9as harvested at the same time post pollination.
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In species that display overlap in phenology, reproductive isolation can be achieved by specific pollinators, post-pollination barriers or a combination of both.
Many studies have measured the interactions among pollinator behavior, floral-display size, and plant reproductive success, but few studies have been able to separate the effects of pollinator behavior and post-pollination processes on angiosperm sexual reproduction.
In Nicotiana attenuata a post-pollination ethylene burst originating from the stigma and upper style in pollinated flowers was associated with both termination of benzylacetone release which attracts pollinators and augmented differences in siring success between pollen genotypes in mixed pollinations (Bhattacharya and Baldwin 2012).
Colle, M. et al. Variation in cucumber (Cucumis sativus L). fruit size and shape results from multiple components acting pre-anthesis and post-pollination.
Both the male and female organs of the flower are especially sensitive to temperature fluctuations both during their development before pollination and during the post-pollination stage.
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