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Patch clamp recordings were performed at 7 14 d post plating.
Neurons were infected with AAV vector containing GFP-Actin monomer 4 h post plating.
The cells retain morphologic and biochemical characteristics of differentiated hepatocytes through day 30 post plating, including liver-specific gene expression.
At day 8 post plating, neuroepithelial clusters were also negative for CD73, however, ∼13% of the migrating emNCSCs were positive for CD73 (Figure 10B C).
We examined CD73 expression in day 5 neurospheres prior to culturing on fibronectin, as well as in epithelial clusters and migratory cells 3 days post plating on fibronectin.
The numbers of spheres per well were counted 7 days post plating and were plotted as the percentage of the number of spheres obtained per 20,000 cells plated per well.
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vSMC plated in monoculture were subjected to MTS assay four days post-plating.
For 2 3 days post-plating the medium included 15%% heat inactivated fetal calf serum.
Calcium studies were performed on differentiated neurons 7 9 days post-plating.
Cells were retreated 3 days post-plating and fixed with 4% paraformaldehyle (Electron Microscopy Sciences) at day 6.
Two counts were performed for each of the three dishes of WT and epsin TKO cells using a hemocytometer, both at 1 and 3 days post-plating.
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