Prior to capacitation, both populations were present, with the acrosomal population being more prominent, and following capacitation, sperm showed post-acrosomal localisation only.
The study evaluates the pre- and post-training lesion localisation ability of a group of novice observers.
TGFβ is regulated on many levels, through intracellular post-translational modification, localisation to the ECM and through a variety of activation mechanisms.
Sequences were submitted to ProtFun 2.2 Server (http://www.cbs.dtu.dk/services/ProtFun/) for analysis of the GO ontology to predict function based on amino acid sequence-derived input features (physical/chemical and functional biological properties) such as predicted protein secondary structure, transmembrane helices, subcellular localisation and post-transcriptional modifications [ 66].
Repeat gamma camera and SPECT scans were performed a week after treatment to assess localisation of I131 KAb201 post-therapy.
Regulation of its activity depends mainly on changes in the subcellular localisation, achieved via post-translational modifications, including phosphorylation, acetylation and ubiquitination (Calnan and Brunet, 2008).
Function of p53 is regulated by several mechanisms, acting not only at the transcriptional and translational level, but also on stability, post-translational modification, and subcellular localisation of p53.
Detailed information on the putative functions of the 185 ORFs, their putative post-translational modifications and cellular localisation are shown in Additional file 4.> To confirm that these DNA fragments belonged to uncultured H. walsbyi and not to other Archaea also present in the brines, we calculated their tetranucleotide frequencies and performed a principle component analysis [ 29].
Cells were subsequently incubated in 500 μl Krebs containing 3 μM ionomycin or 100 μM histamine for required times before either solubilisation in SDS dissociation buffer, to investigate the phosphorylation state of NFAT via Western blotting, or fixing in 4% formaldehyde to observe the resulting sub-cellular localisation of NFAT post-treatment via confocal microscopy.
The hnRNP proteins thus seem to be present throughout the length of pre-mRNPs and on individual transcripts they are likely to be present in specific combinations (reviewed in Singh and Valcárcel 2005), presumably determined by preferential sequence binding properties, post-translational modifications and cellular localisation.
