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The humoral immune response to live and inactivated N. seriolae immunised ginbuna showed high antibody titre at 15th day post immunisation but the level declined subsequently in both the immunised groups.
Inguinal lymph nodes from bCII immunised DBA/1 mice were excised at day 14 post immunisation and cells were dissociated and plated out in a U bottom 96-well plate at 2 × 106 cells/ml in RPMI 1640 containing 10% fetal bovine serum, 50 U/ml penicillin/streptomycin and 50 μM 2-mercaptoethanol.
On the contrary, high percentage of CD4+ T cells was observed in live N. seriolae immunised group in both the head and trunk kidneys at 30th day post immunisation.
Vα/β families with clonal expansions are depicted for post immunisation (left column) and before immunisation (recognized as control, right column).
The vaccine can provide solid protection against virulent strain challenge at 7 days post immunisation (DPI 4 or even earlier5,6,7.
The percentage of CD8α+ T cells in spleen and head kidney of ginbuna was significantly higher at 3rd day post immunisation.
Similar(32)
HI responses were sustained at 3 months post-immunisation in both groups.
Splenocytes were harvested as described [58] seven days post-immunisation for flow cytometric analysis or fourteen days post-immunisation for ELIspot analysis.
The levels of CD8+ T cells induced by this co-immunisation strategy were comparable to those seen with Ad-CMVpoly alone and were detectable at reasonably high levels on day 75 post-immunisation (Figure 3A).
In contrast, the antibody response showed significant increase in the virus neutralization capacity by day 75 post-immunisation (Figure 3C) which was co-incident with the antibody avidity maturation (Figure 3D).
The levels of gB-specific antibody responses were maintained at high levels by day 75 post-immunisation, although a small reduction was observed when compared to the levels observed on days 10 and 25 respectively.
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