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We therefore chose examples of results of ANA indirect immunofluorescence tests of pre-immune and post immune sera from pairs of littermates (Figure 3).
The specificity of the antisera against EhMAPK was analysed by western blot with E. histolytica total cell lysate and purified recombinant EhMAPK with both the pre and the post immune sera.
Figure 3 shows examples of test results for ANA detected by indirect immunofluorescence on HEp-2 cells, in pre-immune and post immune sera from litter-mate pairs representing four GR- two BB- and two CF-immunized animals.
The blots displayed bands at the expected molecular weights of ∼40 kDa and ∼47 kDa respectively by the post immune sera but not by the pre immune sera indicating a clear recognition of the endogenous EhMAPK as well as the purified recombinant EhMAPK (Fig. 1c).
In contrast, any local chimerism established in the peritoneum of RAG mice failed to persist post immune reconstitution, as donor cells were undetectable in all reconstituted RAG recipients.
This protection is evident 6, 12 and 24 hours post immune priming and involves the activation of both the Imd and the Toll pathway.
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The comparative analysis of gene expression post immune-challenge in different developmental stages of C. floridanus suggests a stronger induction of immune gene expression in larvae in comparison to adults.
Fourteen vaccine-induced antigens identified from the pre-post immune screen were examined for their immunoreactivity and antigen-specific antibody titer in sera from 19 healthy donor and 19 CML patients (including five from the initial screening described above) at seven time points during the vaccine trial.
Pre-immune blood samples were obtained 2 days before immunization, whereas post-immune blood samples were drawn and analyzed 5 days after immunization.
The post-immune sera of rabbits immunized with LPS-OH-TT, dLPS-BSA or dLPS-TT displayed significant bactericidal activity against mutant and wild-type α-1,6-glucan-expressing strains and selected clinical isolates of H. pylori.
When mice are passively transferred post-immune serum of OVA-immunized mice, followed by challenge with particulate OVA, this also induces vascular leakage in mice (although unlike DENV and UV-DENV, pOVA does not elevate the vascular leakage at baseline; Figure 5E).
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