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At the end of each treatment, rumen fluid was collected at 3 h post feeding.
On the third collection day, blood samples were drawn at the time of feeding, each hour for five hours post feeding, and eight hours post feeding for serum Cu analysis.
Blood samples were obtained on d 3 and 7 at 2 h post feeding for plasma Ca determination.
The development of third larval instar include feeding and post feeding stages, took 8 and 5 days at the temperatures 15°C and 20°C, respectively.
Samples were collected on the last day (42 days dietary period), 3 hours post feeding using flexible stomach tube and vacuum pump from rumen of the buffalo.
Protozoa population will be low at 3 h post fed sampling time thereby subsequently reducing the proportion of methanomicrobiales (Sahoo et al. 2000).
At 4 h post feeding, the concentration of ruminal ammonia nitrogen decreased with increasing levels of U-cas in feed blocks (P<0.05).
Urine was collected every four hours for 72 hours and multiple fecal grab samples were taken every two hours post feeding.
And as one post fed off another, others began to say with certainty — but again without proof — that Warsame was murdered and "it looks like a cover-up".
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CEO of Professional Science Editing for Scientists @ prosciediting.com