Exact(21)
A low post chip RNA degradation index (3'/5' ratio < 3) corresponds to high quality material while high post chip RNA degradation index (3'/5' ratio > 3) could indicate both low quality material and/or problems encountered during sample processing.
Thus, to characterise post chip RNA quality we use 5'/3' ratio of beta actin and GAPDH genes.
A detailed analysis of effects of post chip RNA quality on the measured abundance of transcripts is presented.
A summary of correcting procedures applied to all 10 considered sample sets is presented in Table 3. Correlations of post chip RNA quality profiles are also shown.
We performed a detailed analysis of effects of post chip RNA quality on the measured abundance of transcripts in post-mortem brain samples.
In current study we revealed the detailed profile of post chip RNA quality effect and investigated its technological and biological origin.
Similar(39)
Butler takes a hardline view and his post chips away at the original article by looking at what's missing – namely: "any quotes from anyone who really thinks this might be a bad thing.
The importance of quality control (QC) measures in microarray studies, including pre-chip (RNA quality of samples) and post-chip outcomes of the data, has been described previously [ 19].
To analyze the methylation density of rDNA precipitated with CTCF antibodies, post-ChIP hydrolysis ('chopping') of DNA was performed using the methylation sensitive enzyme HpaII and its isoschizomer MspI.
The pre- and post-chip quality control (QC) criteria established, included: (1) RNA quality, assessed by RNA Integrity Number (RIN) ≥ 7.0; (2) cRNA transcript integrity, assessed by signal intensity ratio of GAPDH 3' to 5' probe sets ≤ 3.0; and (3) the multi-chip normalization scaling factor ≤ 10.0.
And then Jon Sim reached around Rich Pilon at the right post and chipped a shot past Richter at 16:16.
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