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The mice were treated following 2 3 weeks post cell injection and were evaluated for tumor occurrence 8 weeks after treatment.

For cell-based therapies, early engraftment period of 2 to 5 weeks post cell delivery is the most critical time period [20].

Animals were sacrificed by decapitation at 3, 6, 12, 24, 72, and 120 h post cell-free Hb or vehicle administration.

These were identified histologically at 4 weeks post cell injection (Fig. 9A,B).

Disease progression thereafter is rapid, leading to the peak of the disease ∼96 h post cell injection (Figure 1A).

At day 14 post cell implantation, mean tumor volume had reached 170 mm3 and 24 mice were randomized into three groups.

Measurement of bioluminescent signal from intra-abdominal structures at 1 day post immunization resulted in maximal photon flux in the abdominal region by day 5 post cell transfer.

Beginning on day 14 post cell injection, animals received intraperitoneal (i.p).

Cell colouration changed from pink to blue and then to green post cell death (Additional file 12).

E2 treatment led to a 2.3-fold increase of tumor volume at 8 weeks post cell inoculation.

Three weeks post cell injection, mice were injected with D-luciferin, and imaged using the IVIS200 imaging system.