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For signal generation, MUP was loaded into the recirculator at 1 μL min 1 for 2 min. After the recirculator was sealed, the solution was mixed at 5 Hz and fluorescence images were acquired every 30 s (with the shutter closed between exposures) for 10 min and once again at 15 min. Post assay, the entire device was washed with pH 9.5 Tris as it cooled to room temperature.
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This means that post-assay adjustment procedures using parameters from different populations or at different times may not be valid.
Although it is widely recognised that the BED test can misclassify old infections as recent, there has been renewed confidence in using BED-derived estimate of incidence if a post-assay analytic correction procedure is used [14], [16].
Current guidelines [16] support using a post
The single substrate employed for in-well detection further simplifies post-assay processing and data integration and, thus, reduces the total time required for the process.
(C ) Silver stain of post-assay glutathione-bound GST-WDR5 mutants indicates no protein degradation or loading bias occurred during in vitro binding assay.
For instance, Takashi's group reported that lncRNA Air interacted with the H3K9 histone methyl-transferase G9a by CLIP [ 57], but their data suggest that the post-assay RNA quantity is small and the assay is tedious steps.
By coupling the radioactive Ca2+ accumulation assay in microsomes with quantification of ATP hydrolysis and P i release into the post-assay eluate, SERCA-dependent Ca2+ accumulation in comparison to SERCA activity (ATP hydrolysis) can simultaneously be quantified.
Post-assay manipulation of data was undertaken using the DFS analysis (version 2.5) tool developed (and kindly provided) by Niesen et al. [ 33] (downloadable at ftp://ftp.sgc.ox.ac.uk/pub/biophysics).ac.uk/pub/biophysics
In post adsorption assay, baicalein exhibited potent antiviral activity against DENV-2 with IC50 = 6.46 μg/mL.
Three independent high expression lines were used for the post-germination assay.
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