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Post alignment, the aligned reads were put through de novo transcript assembly and numerous bioinformatics tools, along with some in-house scripts for processing.
Dog transcriptome was reconstructed, using CuffLinks in de novo mode post alignment to CanFam3 genome build.
Post alignment filtering of duplicate reads corrects for biases that can arise from PCR duplication during library preparation.
Tag Files are generated post alignment as a summary of Sequence Files and every Sequence File has a corresponding Tag File.
Post alignment QC included computation of insert size distribution and collecting basic RNA-Seq metrics using functionalities provided by Picard tools.
As part of the blinded post hoc analysis for Down syndrome in our previous report, the z-scores were based on GC normalization and use of a repeat masked reference genome post alignment, in order to more accurately reflect the mappable genome from each chromosome.
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Roy Wade (6-7 senior) will join part of a double-post alignment, and Osei Appiah (6-3 senior) will be at the other forward.
The amount of bits needed to store the SA array (used in the post-alignment stage) is the same for all the aligners.
WES analysis including alignment, post-alignment processing, and variant calling from WES data was performed as described previously44.
Subsequently, it simulates and outputs sequence contigs (FASTAs), sequence reads (FASTQs) and/or post-alignment files (BAMs).
The basic statistical and diversity analyses (calculation of ShannonDI 41 of the T-cell clones were accomplished using a post-alignment analysis tool, VDJtools v1.0.042.
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