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"The draperies were mounted as high as practically possible to span the width of the sliding doors and the grass shade prevents the eye from climbing any higher," he says.
Primers were designed, where possible, to span exons and to generate a fragment of between 80 and 130 bp.
Amplicons were designed, when possible, to span one or more introns to avoid amplification of the target gene in genomic DNA.
Primers were designed (where possible) to span intron/exon boundaries so that amplification of genomic DNA in qRT-PCR could be avoided.
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Primers were designed to amplify single products from the target gene between 140 and 250 bp with an optimal GC content of 50% and, where possible, designed to span an intron to ensure that cDNA targets were amplified.
The main limitation is that it was not possible for queries to span multiple object types.
In the cases of putative superinfections, we attempted to analyze pairs of stored samples dated as close as possible to and spanning the putative event of superinfection.
When possible, primers were designed to span an intron to prevent possible amplification of contaminating genomic DNA.
Where possible, primers were designed to span an exon junction but this was not possible for six of the P450 genes (CYP325A1, CYP6P3, CYP4G17, CYP6Z3, CYP12F2 and CYP6Z2) due to the high degree of polymorphisms in their DNA sequence.
Where possible, primers were designed to span exon junctions to further reduce the possibility of amplification of non-specific products from contaminating genomic DNA.
Where possible, assays were designed to span an intron, and for the majority of the cases this was possible.
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CEO of Professional Science Editing for Scientists @ prosciediting.com