Therefore, it may be possible to develop a functional HC assay in the intact cells that will serve as bases for a future high-throughput screening assay for the discovery of HC blockers.
Using a close relative of M. tuberculosis, Mycobacterium marinum, Stoop et al. develop a medium-throughput screen for virulence factors using M. marinum's natural host, the zebrafish, and identify three novel virulence factors that are involved in granuloma formation.
As a first step to evaluate the tumor reactivity and specificity of patient B cell-derived IgG antibodies, we developed a medium-throughput cell-based ELISA with melanoma cells to detect antibodies against tumor cell surface antigens (Figure 1).
Being able to produce and vial large single lots of Pv spz that can be used in multiple experiments over time is a first prerequisite to developing a medium to high throughput screening assay.
It has been possible to develop high throughput methods that were capable of measuring telomere length of individual cells (Flow FISH and HT Q-FISH, Figure 7), but individual telomere quantification remained for years an exclusive property of metaphase Q-FISH.
In order to test our hypothesis that the cells that grow out after 20 days are those in which EZH2 has either not been targeted, or in which the indels lead to in-frame alterations, we developed a medium throughput assay to analyze 10s 100s of clones for gene essentiality.
Hence a stable, clonal cell line containing a light detection sensor and a receptor, which is generated from incorporating the respective genetic material, become critical considerations in developing a medium throughput assay for selective receptor activation.
We have developed a medium throughput phenotypic assay, based on the identification of nuclear foci in DM patient cell lines using in situ hybridization and high-content imaging to screen for potentially useful therapeutic compounds.
Such representation of the medium by objects makes it possible to develop realistic tissue models presenting 3D spatial variations of a biological structure.
The availability of a large expressed sequence tags (EST) resource and recent advances in high-throughput genotyping technology have made it possible to develop highly multiplexed SNP arrays for multi-objective genetic applications, including the construction of meiotic maps.
