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Two sets of subjects with alternation between fluid microinjection and mock microinjection were used, so that the total number of microinjections was limited to four, thereby minimizing possible tissue damage and loss of sensitivity at the microinjection site65.
To evaluate possible tissue damage caused by the generated heat increase, cumulative equivalent minutes (CEM43) were calculated and it was found that the combination of small interval length (0.5 mm), high irrigation rate (30 ml/min) and the newly designed drill bit was the only parameter combination which allowed drilling below the time-thermal threshold for tissue damage.
In order to optogenetically investigate the large and complex primate brain, an optical-/electrical probe, or "optrode", must be inserted deeply, which requires the optrode to be not only long and stiff, but also sharp and smooth to reduce possible tissue damage.
These parameters are below the threshold for possible tissue damage [14].
The cut-off time was set at 30 seconds for thermal and 100 seconds for cold allodynia to prevent possible tissue damage to the affected paw.
To determine the mechanisms by which GP526 affected the adult worm motility, we evaluated the possible tissue damage induced by B. thuringiensis, analyzing histological sections of D. Caninum adults.
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It is possible that a thermal stimulus of higher temperature would have decreased the cooling component even further; however lingual application was not possible because of the potential for tissue damage.
The observed changes in the normal balance of the circulating mediators of inflammation and their possible association with tissue damage, oxidative stress, and post-race susceptibility to infections warrant further studies in larger populations.
The observed changes in the normal balance of the circulating mediators of inflammation and their possible association with tissue damage, oxidative stress, and functioning of the immune system warrant further study in larger numbers.
The level of m-THPC in other tissues is also important in PDT to predict the possible extent of normal tissue damage.
Therefore, the possible explanation of extensive tissue damage and mortality that happened in MLKL or RIPK3 mice could be due to exaggerated nature of other types of cell-death pathways.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com