Secondly, the search for the possible specific PS-binding sites on the molecular surface of CT 4 was performed using two independent molecular modeling techniques, namely, molecular docking and molecular dynamics (MD).
The purpose of this study was to investigate, in human skin, the possible existence of specific binding sites that mediate the protective action of resveratrol.
In a study, Bastianetto et al. [ 131] aimed to investigate the possible existence of specific binding sites in skin that mediate the protective action of resveratrol.
Considering the possible non-specific binding of the enzyme and proteins in blood, which may influence the specific analysis, we used BSA to check this effect.
To further investigate the possible un-specific binding between the individual protein with human immunoglobulins, recombinant proteins of Sjc23-LED, GST and TSP-2 of S. japonicum were generated.
Triton 0.3% - BSA 5% was used to block possible non-specific binding before incubation with the Alexa 488-conjugated anti-MOMA-2 monoclonal antibody (Serotec, USA) overnight at 4°C in a humid chamber.
The possible non-specific binding of OTA to the unblocked bead surface was tested.
Two possible explanations for the specific binding of SeMV MP to the genomic RNA are- i) sequence specific interaction of MP with the genome or, ii) direct interaction of MP with the VPg covalently linked to the 5'end of the RNA.
We believe that a more rigorous test of site specific binding will be possible once we develop imaging methods that can unambiguously detect, identify and track individual gene/promoter loci using strategies such as CRISPR/Cas9 tagging of targeted genes/alleles in the genome in live cells.
Each MHC class I molecule, whose total number surpasses the thousands of alleles to date [5], is characterized by a specific binding motif that is possible to "decode".
