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In the middle of the room, a bright point light source is positioned, creating a dynamic range of 11 stops (i.e. the ratio of the brightest and smallest possible pixel intensity is about 211).
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The maximum number of possible patterns for 4 (2×2) tiles was 34 = 81, which from now on will be referred to as P. A small number of pixels and pixel intensities is beneficial for decoding because we can map the cortical response to all possible pixel combinations.
Background-corrected peak pixel intensity (PPIcorr) and rate of change in pixel intensity (PPIcorr/TPPI) were determined for each injection.
The average pixel intensity was determined.
Background pixel intensity of each image was subtracted from pixel intensity of the spot.
In VAPP58S expressing animals, pixel intensity was 58.39±6.60 (mean±SEM).
Only clusters with average pixel intensity 1.5 times greater than background pixel intensity were used for analysis.
The average pixel intensity of the black level measured in the SLR-based system was 2.3 and 5.6 in the CCD-based system; it is not possible to manually adjust the offset level in either system.
The intensity of each spot was summarized as the median pixel intensity followed by log transformation.
The total pixel intensity for clustered ß-DG was acquired as 'circ spot total intensity ch2'.
GenePix 6.1 calculates SNR as the ratio of the mean net signal intensity (mean background pixel intensity subtracted from the mean foreground pixel intensity) over the standard deviation of the background pixel intensity.
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