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This is one important step in the direction towards possible localisation.
11 Anatomical studies of vagal afferents have revealed expression of metabotropic glutamate receptors (mGluRs), including mGluR5, in the nodose ganglia of several species, including humans, and evidence suggests possible localisation in peripheral gastric vagal afferent terminals.
To evaluate the possible localisation of Foscan® in the Golgi apparatus/ER complex, Foscan®-loaded cells were incubated with BPC. Figure 4a shows that the fluorescence spectrum of BPC in MCF-7 cells has two maxima, at 545 and 621 nm.
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In addition, the evidence that some viral infections may have salivary glands as one of the possible localisations, and that focal sialoadenitis may be the result of the local pathological process, again supports the theory - often proposed, but never completely proven - that some viral agent(s) could trigger the immunopathological cascade in pSS [ 15].
We also searched all cystatin sequences for signal peptides indicating their possible cellular localisation (Additional file 2).
We explored its possible co-localisation with cell markers for Golgi, e.r. and mitochondria, and found no convincing co-localisation (not shown).
Using immunohistochemistry we determined the area density of proliferating cells within the SGZ of the DG, measured by the presence of phosphorylated Histone H3 (HH3), and their possible co-localisation with GFAP to exclude a glial phenotype.
This prompted us to investigate the possible co-localisation of Mb and FASN in both healthy and cancerous breast tissue.
It would have been possible wherever the localisation of the infection.
Therefore both staining patterns were evaluated separately to identify a possible cell specific localisation of EGFR and to evaluate an association of a specific pattern with prognostic pathological parameters.
While replacing mCherry with TdTomato, we destroyed a Kozak consensus sequence between the myristoylation sequence and the initiation codon of mCherry, in order to increase the probability that translation would initiate at another Kozak site upstream of the two myristoylation sequences, thereby reducing possible cytoplasmic 'background' localisation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com