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These and other double dye studies are capable of screening NDS and other compounds at the cellular level for a possible cell death method before endangering animal or human lives.
In addition to differential dissolution, internalized NMs can induce cell death in cancer cells by activating all possible cell death pathways (apoptosis, necroptosis or autophagy) either alone or in a synergistic manner.
Therefore, the aim of the present study was to achieve a precise characterization of the cytotoxic and genotoxic activities of this nitroimidazole in cultured human lymphocytes at therapeutic concentrations (0.1, 1, 10 and 50 μg/ml of culture) and evaluate the possible cell death mechanism associated with it.
We also allowed up to 24 hours ex vivo for possible cell death and DNA cleavage to proceed before TUNEL staining, and observed similar results (Figure S3).
Ultimately, the most critical feature of protein quality control systems is that they are required to protect cells during proteotoxic stress and prevent possible cell death.
There was statistically a significant difference (p < 0.05) in hypodiploid cells (possible cell death) at 72 h between the irradiated and control groups (8.48 ± 1.40% and 4.26 ± 0.60%).
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Certain genes were selected and verified by real-time quantitative PCR and, of these genes, robust suppression of Ephrin B3 expression was suggested as a possible cell death-inducing mechanism of combined treatment with IR and PKC 412.
The presence of more than 60% of the proteins in the secretomes without predicted secretion signals indicates possible cell lysis, cell death or non-classic secretory mechanisms.
When DNA repair is not possible, programmed cell death or apoptosis follows.
It is, therefore, possible that cell death of any of these cells contributes to the epidermal hyperplasia.
The third possible configuration, cell death, is not modeled as there is no cell at time t + 1 to model the dynamics; however, the occurrence of timing and cell death is accounted for in the time to event component.
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