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The strength of this technique lies in the possibility to screen a large number of samples within a short time at low costs.
Indeed the pharmacophore notion provides a powerful way to identify and compare structural features across a large set of molecules and the possibility to screen virtually millions of compounds.
The possibility to screen for and quantify the 19 most commonly prescribed neuroleptics on the Swedish market and determine the presence of their major metabolites within a single LC MS MS analysis was evaluated on a PE Sciex API2000 instrument.
In this context transcriptomic approaches associated to toxicogenomic database analysis give the possibility to screen, annotate and cluster high numbers of genes and to identify the molecular changes that univocally mark the toxicity induced processes or are indicative of the early initiating events that lead to cellular toxicity.
Targeted enriched cDNA libraries have also been shown to be an effective method for expression profiling [1], [2], [3] where massive sequencing could be used as readout with the possibility to screen thousands of samples in parallel.
The possibility to screen, localize, and characterize the whole human genome for sequences bound to HMGA2, can help to understand in which way HMGA2 is associated with different biological processes.
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Furthermore, this application opens up novel possibilities to screen for fission yeast mutants with deficiencies in cell-cycle regulation, i.e. where the wanted phenotype involves arrest in a specific cell-cycle phase.
However, the recent development of DArT markers in H. chilense offers new possibilities to screen large number of accessions more efficiently.
The number of possibilities to screen the complete data set is extremely large (in excess of 10) and hence, screening one million random combinations does not guarantee to find the minimal set.
This way, we had the possibility to quickly screen 234,095 patients admitted during 2003 through 2007, but only 704 of that number required a more detailed review.
However, a precondition for these studies is the possibility to accurately screen the phenotypes of transgenic mouse lines in order to identify those defects that are associated with embryonic and pre- or perinatal lethality.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com