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To investigate this possibility, samples of currently used orthodontic nickel-titanium wires (Sentalloy, GAC; Copper Ni-Ti superelastic at 27°C, 35°C, 40oC, Ormco; Nitinol Heat-Activated, 3M-Unitek) were subjected to temperature cycles ranging between 4°C and 60oC.
To definitely exclude this possibility samples that were not separated by MT-G FACS (n = 4) were pre-incubated with DNase or H2O2, or subjected to high temperature in order to effectively damage sperm chromatin using different agents, as previously shown [46].
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One possible problem is the possibility that samples from the patients might have been contaminated with the algae virus.
This opens the possibility that samples from both cheeks would be required to insure that every sample was collected in a study.
To avoid the possibility of sampling clonemates, corals sampled were separated by at least 10 meters.
Although biogenic gas from contamination is always a possibility, the samples were fresh shales in which internal contamination would seem improbable.
The most obvious of which is the possibility that samples taken from the cell lines themselves are not representative of what appears in the serum.
There was a possibility of samples being damaged during the cutting process.
We have considered the possibility that samples from the bleeding sites might not represent a complete picture of the microbiome of unhealthy gum.
To test for this possibility, dried samples of the 4-min pretreated chips were washed with hot water to remove soluble sugars.
However, in our study, five of the D3met tumours showed chromosome 8 alterations, excluding the possibility of sampling of normal cells in at least these samples.
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