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The first possibility pathway (path 1) involves the addition of CO2 to the secondary amine in AEEA, with simultaneous dehydration to form HEIA and water molecule with an energy barrier of −2.15 kcal/mol.
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To further examine such possibilities, pathway-specific assays would need to utilized.
Another exciting possibility: The pathway HIV usurps seems to be important for other viruses as well.
To further solidify the possibility of pathway crosstalk between eHsp90 signaling and EphA2, we evaluated whether NPGA specifically impacted upon EphA2 activation.
To interrogate the possibility of pathway 2, we alternatively combined DEA with Cu OAc 2 in the presence of K3PO4 in DMF at 100 °C (Scheme 4).
This opens the possibility for pathway information to be accessed by other software tools for data visualization, computational analysis and the interpretation of large-scale experimental data.
This analysis also ruled out the possibility that pathways are inserted in different genomic contexts, which would be clearly revealed by positive amplification with the pathway internal primers and negative amplification with the flanking primers.
The possibilities of pathway engineering have shown their strength already in other microorganisms, like E. coli and Saccharomyces cerevisiae.
However, there is still the possibility that pathways other than MEK may mediate the activation of ERK1/2 by n-3 PUFAs.
Conversely, we considered the possibility that pathways may also be differentially regulated following carboplatin exposure, which might not be captured by the behavior of individual genes.
While our studies of NRP-1 and MCL-1 have only yielded very preliminary data in one cell line, the possibility of pathways linking NRP-1, signal transduction, antiapoptotic proteins, and cell survival are intriguing.
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