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Badakere et al. [ 104] and Kulkarni et al. [ 89] reported the possibility of staining RBCs, WBCs, platelets, lymphocytes, nucleated cells, and genomic DNA with C-PC.
In contrast, reduced pigment content in any part of the fruit has been the aim of most breeding programs for the canning industry because of the possibility of staining in the processed product.
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In ex vivo tissue the possibility of cross staining endogenous E. coli was excluded by ensuring that matched, adjacent tissue (from the same patient) was completely negative for E. coli via immunofluorescence.
For example, Wang et al. (2016) have demonstrated the possibility of direct staining and imaging of mouse HER-2 positive breast tumor tissues by applying SEPs functionalized with anti-HER2, followed by a rapid rinse with serum to remove unspecifically deposited SEPs (Fig. 5).
Three "positive" controls were performed to minimize the possibility of artifactual staining.
We recommend that pathologists not only be aware of the possibility of nonspecific staining but also train during validation of the IHC testing to correctly identify such cases and avoid misinterpretation of nonspecific staining as positive staining.
The anti-cytokeratin Mab, Cam 5.2, was used to identify tumour (and normal) epithelial cells in the adenocarcinomas, to exclude the possibility of CD1a staining of epithelial cells as opposed to DC (Makin et al, 1984; Hillenbrand et al, 1999).
Several groups have explored the possibility of using histochemically stained slides for DNA extraction and have reported adverse consequences of the staining on DNA amplification [ 14- 18].
To investigate these possibilities, we performed staining of Ki67 (supplementary material Fig. S3A) and a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay (supplementary material Fig. S3B) on P7 littermates.
Note that the level of CD31 expression was proportional to that of CD45 expression (Fig. 3C), and the isotype antibody control excluded the possibility of non-specific staining.
However, possibility of inadvertent vitreous staining especially in a case of traumatic cataract with compromised zonular apparatus should be kept in mind[ 21].
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