They showed the possibility of quantification of the concentration of the charged protein molecules in liquid solution, but did not report an application with repeatable consecutive measurements.
After verifying the utility of MMIHC on a chip and examining repeatability and the possibility of quantification using cell blocks, we applied the platform to patient tumor tissues which are heterogeneous in terms of morphology, genetic alterations and histopathological features.
Their pilot study suggested possibility of quantification of distribution of micro- and nanosized particles in tissue samples.
Furthermore, this probe melting point analysis revealed the possibility of quantification detection with 10-fold diluted synthetic DNA targets (10 to 1 start copies) (Fig. 7b).
This pilot study has proven the possibility of quantification of distribution of micro- and nanosized particles in tissue samples, which had previously been one of the main challenges of the clinical research in nanopathology.
Hence, hyperpolarized C enables non-invasive, in vivo and real time assessment of metabolic processes, with the possibility of quantification, e.g. enzyme-mediated flux of metabolite intermediates (PDH, LDH (l-lactate dehydrogenase), CA).
