However, an important pitfall of this approach is the possibility of phenotype reversion in G-CSF-induced DCs, leading to the opposite effect of inducing immunity.
As different ciliopathy protein complexes can be found in different subcompartments of this cellular organelle, such a concept includes the possibility of differential phenotypes according to the different affected subparts of the cilium.
The advantage of this strategy is that the nearly infinite possibility of created phenotypes can effectively be studied because a selective strategy is being used.
As a corollary, one likewise does not need to reckon with the possibility of mutant phenotypes in learning actually reflecting deficiencies in social interaction.
Moreover, one of the interesting benefits specific to this study was the possibility of linking phenotypes that were recorded exclusively in A × B individuals to genotypes available only for parents and grand-parents in some cases.
Another limitation is the possibility of different phenotypes in osteoarthritis, 1 suggesting that strontium ranelate might be more effective in certain subgroups of osteoarthritis patients, for example, those with dominant subchondral bone changes.
However, to avoid the possibility of confounding phenotypes, we used only the alleles l(3)70Da s4868 and l(3)70Da, which harbor smaller mutations affecting only the 5′ region of the Pex1 gene, for subsequent developmental characterizations.
To rule out the possibility of additive phenotypes or redundancy between the different Type I systems, we constructed a toaD/tobD/prsD triple mutant (A912) and toaD/prsD (A911) and tobD/prsD (A903) double mutants, but all had the same phenotypes as A898 (prsD).
A great advantage of flow cytometry is the possibility of simultaneous phenotype characterization of antigen-specific T cell by multicolour flow cytometry.
Although clinical adjudication represents the best possibility of accurately phenotyping patients, the true gold standard to assess degree of renal injury would be through biopsy.
