The results from our retrotransposon screen suggest that aside from a possibility of lack of data, it might be not possible to find a single sistergroup to Notoryctemorphia due to the short divergence times between the Australian marsupial orders.
Patients who responded to medical treatments, pregnants, symptomatic TN (abnormal neurological examination or demonstrable structural lesion by MRI) and patient with possibility of lack of coherence during follow up.
Although this lack of differences strengthens confidence in the approach, to further address the possibility of lack of comparability we conducted all analyses controlling for undergraduate GPA.
Firstly, the power of our case-control study only reached 12.2% at alpha level of 0.05, so we could not exclude the possibility of lack of power in our study mainly due to the relatively small sample size.
Therefore there is some possibility of lack of independence for a (probably) small portion of our data points that might have been collected from nests of the same birds.
The small sample size and the possibility of lack of adjustment for unknown/unconcontrolled confounders as well as some biases like recall (questionnaires reporting) or healthy worker effect (mainly among sewage workers) could also be considered as one of the study limitations.
However, we cannot exclude the possibility of effectively lacking further risk increase in highly exposed workers.
Although electronic pacemakers are an excellent therapy, their persistent shortcomings (need for monitoring and replacement, indwelling catheter electrodes in the heart, possibility of infection, lack of autonomic responsiveness, geometric limitations with respect to pediatric patients) are sufficient to warrant a search for better alternatives.
Our observation of a previously vaccinated boy developing JE from genotype 1 virus infection also calls for more detailed studies, both in vitro and in vivo neutralization tests as well as active surveillance, to examine the possibility of a lack of complete protection conferred by the live attenuated JE vaccine against genotype 1 virus.
To rule out the possibility of a lack of the ALC population as a whole in the rblA disruptant we stained with neutral red Pbtg-αGal slugs of both AX2 and rblA KO cells and observed that the total population of ALC in the rblA disruptant is not decreased (Fig. S3).
