To assess the latter possibility, expression of pocket proteins (pRb, p107, and p130) were examined.
To investigate this possibility, expression levels of the ΔNPEA3En transgene in mammary glands and tumors were measured by RT-PCR.
To test this possibility, expression of brk was examined in dTIEGS14/Minute clones and compared to tkva12/Minute clones.
To examine this possibility, expression and methylation analyses were performed on two of the cancer related genes (Figures 4 and 5).
In line with this possibility, expression of an extra class of small RNAs molecules involved in gene silencing, so called piRNA, is expressed specifically in spermatogenic cells [ 59].
To explore this possibility, expression data from this cohort were analysed for associations between expression of EMMPRIN and S100A4 in primary tumour samples, and functional studies were carried out in two colorectal cancer cell lines.
Consistent with this possibility, expression of GFP-OCRLWT suppressed the comets, while expression of catalytically-inactive GFP-OCRLD523G localized to the head of the comets but failed to rescue this phenotype.
Considering these three possibilities, expression of each gene was classified into 3×3×3=27 patterns, 1− D D D,2− D D S,⋯,27− I I I, where the order of the letters corresponds to the transition.
(Dickinson had hinted at such a possibility with expressions of pain that were obviously sincere).
The expression observed in empty pcDNA3.1+ transfected cells raised the possibility that expression may be induced by the transfection and selection process.
