The responses were differentially recorded from FZ, TL and TR (positive) with reference to nose tip (negative) using silver chloride electrodes.
In detecting ILTV, the LAMP assay detected all 5 strains previously isolated, did not cross-react with other avian pathogens, and obtained a 100% sensitivity in 43 positive clinical samples with reference to virus isolation.
The percentage of test cells that incorporated EdU was determined by gating on the NucLight Red positive cell population and applying an EdU-positive gate set with reference to negative control (carrier) cells processed with the Click-iT EdU assay but not previously exposed to an EdU pulse.
Therefore, a scheme is not given, and positions are described with reference to the Fas2-positive tracts in a8.
In many cancer studies, including those related to breast cancer, a negative and positive correlation are described with reference to high or low maspin expression level as a prognostic factor of tumor development [ 16- 19].
Local activation of the remaining bipolar electrograms was defined automatically from the maximum peak positive or negative deflection with reference to the isoelectric baseline after which far-field ventricular activation was identified and excluded from the analysis of local activation.
Growth inhibition was calculated with reference to positive control.
For both PTEN and pAKT, the percentage of cells expressing antigens was determined by evaluating the number of positive cells in a field with reference to the total number of cells in that field.
Positive cell populations were gated with reference to negative isotype control matched antibody staining reactions (Mouse IgG1 PE and APC, Cat. No. 559320 and 554681, respectively; Mouse IgG2a FITC, Cat. No. 553456; BD Biosciences) and baseline HER2 expression on CD14+, CD56+, CD19+, and CD3+ cells from normal healthy volunteer PBMCs (without cancer cell co-culture).
The trepidation inherent in the case law on positive obligations can also be explained with reference to the political backlash that would arise from imposing positive duties on Member States.
Although 10% positive feedback may be more "realistic" with reference to the perceived difficulty of the task (only one participant in the 10% group realised that the feedback was given at less than chance level), 33% positive feedback yielded more learning.
