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Sections known to stain positively were used as positive controls.
The cases with ⩾10% carcinoma cells stained positively were classified as positive.
Tissue sections previously stained positively were used as positive control, while tissue sections with primary antibodies replaced by phosphate-buffered saline served as negative control.
Tissue sections with macrophages previously stained positively were used as positive control, while tissue sections with primary antibodies replaced by mouse isotype IgG1 served as negative control.
Sections known to stain positively were included as negative and positive controls.
Sections known to be stained positively were included in each run as positive staining controls.
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