M-hMPV protein was detected from supernatant of hMPV infected Vero cells using positively charged magnetic beads obtained from Dr. R. Veyret, bioMérieux, Lyon, France [23].
CD14+ monocytes were positively selected with magnetic-activated cell sorting (MACS) magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's protocol.
CD62Lhi cells were then positively isolated by magnetic cell sorting.
CD4+-T cells were positively selected with magnetic beads and subsequently sorted with autoMACS (Miltenyi Biotec, Bergisch Gladbach, Germany).
CD8+ T lymphocytes were positively selected by magnetic cell sorting from PBL using anti-CD8 mAb, then expanded in vitro under nonspecific stimulation in IL-2/CM supplemented with leukoagglutinin at 1 mg/mL, irradiated allogeneic PBL and LCL as described [39].
Labelled cells were positively selected on magnetic cell separation (MACS) columns (Miltenyi Biotec) and subsequently labelled with antibodies against CD4 (GK1.5; Pharmingen) and CD8 53-6.7 53-6.7hern Biotechnology ASouthern Biotechnology Associates
CLL samples were positively enriched by magnetic cell sorting using CD19 microbeads and MACS columns (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and resuspended in RLT buffer (Qiagen, Crawley, West Sussex, UK).
Primary CD4+T, CD8+ T, CD56+ NK, CD19+ B cells and CD14+ monocytes were positively sorted by magnetic beads according to the manufacturer's instructions (Miltenyi Biotec, Bergisch Gladbach, Germany).
CD14 cells were positively selected by magnetic-associated cell sorting (AutoMACS: program possel; Miltenyi Biotec).
