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In order to confirm this finding, we decided to positively label the otd −/− LALv1 neurons and in this background assay the activity of the Gal4 drivers.
In the following dual MARCM experiments, we used the GH146-LexA to positively label the transformed otd −/− LALv1 neurons and combined it with either OK371- Gal4 or Per- Gal4 driver lines to assay for their activity in the transformed neurons.
Furthermore, we observed that cell death markers, including acridine orange (AO) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), could positively label many cells in the larval eye discs, and their staining patterns were unaltered by GMR-P35.
(E – H and I – L ) document otd −/− LALv1 neuroblast clones that have been dual labelled with GH146-LexA (E and I ) to positively label the mutant neurons and either OK371-Gal4 (E – H ) or Per-Gal4 (I – L ) using the dual MARCM technique.
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Cell extracts were prepared from untransfected CHO cells (negative control) or from cells exhibiting stable expression of YFP (~73.4±0.6% positively labelled), or else YFP PLCζ (97.6±0.1% positively labelled).
There was little difference in prediction accuracy for semi-supervised methods trained on positively labeled data only, compared with training on positive and negative samples.
Sentiment analysis can however be useful to highlight storylines from highly positive or negative sentiment tweets as possible candidates for positively labeled training data for deteriorating or improving perception bands to reduce analyst's workload.
Both H&P staining and DING protein immunochemistry were combined on same brain sections (co-labeling experiments) to ensure that all brain nuclei are positively labeled with anti-DING antibody (data not shown).
BrdU incorporation studies and immune-labeling of Ki67 and pH3 showed a dramatic reduction in positively labeled cells in all three regional organoids after IFNβ treatment (Fig. 5c f, S3D, S3E).
Cell sorting was performed by autoMACS (Miltenyi Biotec), and later, the CD133 positively labeled with the magnetic microbeads were separated to obtain the positive fraction as previously described (Ohtsuki et al, 2007).
Immunohistochemistry signals for macrophages and KI-67-positive cells were quantified in the alveolar space by counting the number of positively labeled cells normalized by the total number of cells with hematoxylin-stained nuclei.
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