Strong positive staining results confirmed that rhBMP-2 released from the scaffold could improve osteocalcin (OCN) and alkaline phosphatase (ALP) expression and calcium deposition formation.
Primary antibody negative controls and CYP450 antibody positive staining results clearly indicated that CYP2J2 and CYP2Cs proteins were expressed in human cardiomyocytes (Figure 4).
Positive staining resulted in brownish color, negative controls as well as unstained cells in blue color.
Positive staining resulted in a brownish color, negative controls and unstained cells displayed a blue color.
These results suggest that the frequency of FGFR2 amplification is around 5%, which is lower than the positive staining results obtained using immunohistochemistry.
For EGFR, membranous staining was scored according to the guidelines in the Dako PharmDx kit and a SI was calculated when this was combined with the proportion of cells showing positive staining resulting in a SI as described above.
Non-specificity of an immunohistochemical stain may lead to a false positive stain, resulting in a report that the molecule is expressed in that tissue, whereas, in reality, it is not.
All ETB patients were diagnosed by clinical examination, radiological imaging or a positive histopathological staining result suggestive of granulomatous inflammation of site specific biopsy or FNAC (fine needle aspirate cytology).
3- 4,5-Dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT) toxicity data also revealed a slight reduction in cell metabolic activity when PBS was used as the incubation medium (at 50 μ m of 1⋅Ln), supporting the positive PI staining result.
Interestingly, patients who had 'positive' staining results for both activated STAT3 and STAT5 (33 patients) had significantly better overall survival and disease-free survival than those are 'negative' for both staining (13 patients).
Negative staining in the absence of internal positive control staining resulted in exclusion of the result for further analysis.
