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b PLA signal counts/cell (relative EGFR expression level) in EGFR positive sections vs. volumes of the excised tumors.
The basic algorithm contains no randomization and is based on two SVD processes, one approximating the data matrix, the other approximating positive sections of the resulting partial SVD factors utilizing an algebraic property of unit rank matrices.
Numbers of positive sections and immunofluorescence scores were higher in the lungs than in the upper respiratory tract (Table 6).
For each sample, the number of positive sections was counted and the number of fluorescing cells in the epithelium was scored (Table 7).
To get an estimation of the amount of trangene expressing cells over eighty GFP positive sections taken from six different animals sacrificed 2 days post electroporation (dpe) were quantitatively evaluated.
Representations of positive sections (Fig. 1b, left panel) and negative sections (Fig. 1b, right panel) are shown.
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In the positive section the cell membrane and/or cytoplasm were clear brown-yellowish in color.
For each positive section, four microscopic fields showing immunopositivity were selected and photomicrographs were captured at a magnification of 20×.
This result, taken together with robust detection of positive selection (Table 2), suggests that positive section was reliably identified from our ten samples of streptococci gene sequences.
However, the mechanism may not be straight-forward, with some deleterious mutations being selected against (purifying selection), and some possibly undergoing positive section [24].
If cytokeratin deposits were detected, the positive section was compared with the immediately adjacent serial section previously stained with H&E to determine whether the positivity was due to the presence of viable tumour cells.
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